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pcdna1 flag atxn1 cag 85  (Addgene inc)


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    Structured Review

    Addgene inc pcdna1 flag atxn1 cag 85
    Pcdna1 Flag Atxn1 Cag 85, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna1+flag/pcDNA1+Flag+ATXN1%5B85Q%5D+(Plasmid+%2333237)/pm41422503-878-16-18
    Average 91 stars, based on 4 article reviews
    pcdna1 flag atxn1 cag 85 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease
    Article Snippet: .. pcDNA1 Flag ATXN1[30Q] , Skinner et al. , Addgene plasmid #33236. .. pcDNA1 Flag ATXN1[85Q] , Skinner et al. , Addgene plasmid #33237.

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease
    Article Snippet: pcDNA1 Flag ATXN1[30Q] , Skinner et al. , Addgene plasmid #33236. .. pcDNA1 Flag ATXN1[85Q] , Skinner et al. , Addgene plasmid #33237. ..

    Article Title: Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
    Article Snippet: .. After 24 h, HeLa cells were transfected with DNA plasmids containing FLAG‐tagged ataxin1 (ATXN1) with either a normal CAG repeat tract (pcDNA1 Flag ATXN1[30Q]) or a pathologically expanded CAG repeat tract (pcDNA1 Flag ATXN1[85Q]). pcDNA1 Flag ATXN1[30Q] and pcDNA1 Flag ATXN1[85Q] were kind gifts from Huda Zoghbi ( ) (Addgene plasmid #33236 and #33237, respectively). .. Transfections were carried out in Opti‐MEM reduced serum media (Thermo Fisher Scientific), using FuGENE 6 (Promega) transfection reagent according to the manufacturer’s instructions.

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease
    Article Snippet: For the SCA1 experiment, PRT4165 (Sigma #SML1013) was identified as a PRC1 inhibitor using the Drug Repurposing Hub. .. Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq. .. All cells were collected using the QIAGEN miRNeasy Mini or Micro Kit with on-column DNase digestion.

    Transfection:

    Article Title: Abnormal scaffold attachment factor 1 expression and localization in spinocerebellar ataxias and Huntington’s chorea
    Article Snippet: .. After 24 h, HeLa cells were transfected with DNA plasmids containing FLAG‐tagged ataxin1 (ATXN1) with either a normal CAG repeat tract (pcDNA1 Flag ATXN1[30Q]) or a pathologically expanded CAG repeat tract (pcDNA1 Flag ATXN1[85Q]). pcDNA1 Flag ATXN1[30Q] and pcDNA1 Flag ATXN1[85Q] were kind gifts from Huda Zoghbi ( ) (Addgene plasmid #33236 and #33237, respectively). .. Transfections were carried out in Opti‐MEM reduced serum media (Thermo Fisher Scientific), using FuGENE 6 (Promega) transfection reagent according to the manufacturer’s instructions.

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease
    Article Snippet: For the SCA1 experiment, PRT4165 (Sigma #SML1013) was identified as a PRC1 inhibitor using the Drug Repurposing Hub. .. Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq. .. All cells were collected using the QIAGEN miRNeasy Mini or Micro Kit with on-column DNase digestion.

    Cell Culture:

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease
    Article Snippet: For the SCA1 experiment, PRT4165 (Sigma #SML1013) was identified as a PRC1 inhibitor using the Drug Repurposing Hub. .. Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq. .. All cells were collected using the QIAGEN miRNeasy Mini or Micro Kit with on-column DNase digestion.

    RNA Sequencing:

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease
    Article Snippet: For the SCA1 experiment, PRT4165 (Sigma #SML1013) was identified as a PRC1 inhibitor using the Drug Repurposing Hub. .. Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq. .. All cells were collected using the QIAGEN miRNeasy Mini or Micro Kit with on-column DNase digestion.



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    Image Search Results


    Journal: iScience

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease

    doi: 10.1016/j.isci.2023.106358

    Figure Lengend Snippet:

    Article Snippet: Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq.

    Techniques: Sample Prep, Plasmid Preparation, Software

    Journal: iScience

    Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease

    doi: 10.1016/j.isci.2023.106358

    Figure Lengend Snippet:

    Article Snippet: Cells were first lipofectamine transfected with 2.5 μg of either the pcDNA1 Flag ATXN1[30Q] (addgene #33236) or pcDNA1 Flag ATXN1[85Q] plasmid (addgene #33237), cultured in serum-free media with 0.05 mM PRT4165 or vehicle (0.06% DMSO) for 48 hours, and then processed for RNA-seq.

    Techniques: Sample Prep, Plasmid Preparation, Software